LOCUS       40924_25646       13417 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Leishmania vector pIR3HYG-GW (a)-GFP+ (b), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13417)
  AUTHORS   Brettmann EA, Lye LF, Beverley SM.
  TITLE     Spontaneous excision and facilitated recovery as a control for 
            phenotypes arising from RNA interference and other dominant 
            transgenes
  JOURNAL   Mol. Biochem. Parasitol. (2018) In press
  PUBMED    29357296
REFERENCE   2  (bases 1 to 13417)
  AUTHORS   Brettmann EA, Beverley SM, Lye L-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-DEC-2017) Department of Molecular Microbiology, 
            Washington University School of Medicine, 660 S. Euclid Ave, Campus 
            Box 8230, St. Louis, MO 63110, USA
REFERENCE   3  (bases 1 to 13417)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13417)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Biochem. Parasitol. (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-DEC-2017) Department of Molecular Microbiology, Washington 
            University School of Medicine, 660 S. Euclid Ave, Campus Box 8230, 
            St. Louis, MO 63110, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Lasergene v. 12
            Sequencing Technology :: Sanger dideoxy sequencing; 454 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    25..149
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(193..495)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(840..1496)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(1550..1580)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(1610..1729)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    1741..2189
                     /label=MusPEX11- MusPEX11 loop sequences
                     /note="MusPEX11- MusPEX11 loop sequences"
     CDS             2204..2233
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             2240..2269
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2276..2305
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    2321..2440
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2470..2500
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2554..3210
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3555..3857
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3901..4025)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(4227..4245)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    4322..5043
                     /note="alpha tub IR- Leishmania braziliensis strain M2903 
                     alpha-tubulin intergenic region"
     misc_feature    4973..4974
                     /label=SA- spice acceptor AG dinucleotide
                     /note="SA- spice acceptor AG dinucleotide"
     CDS             5062..5778
                     /label=EGFP
                     /note="enhanced GFP"
     promoter        complement(5974..5992)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    6059..6607
                     /note="LPG1 IR- Leishmania donovani strain 1S2D LPG1
                     intergenic region"
     misc_feature    6561..6562
                     /label=SA- spice acceptor AG dinucleotide
                     /note="SA- spice acceptor AG dinucleotide"
     RBS             6619..6641
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             6667..7689
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     misc_feature    7728..8299
                     /note="1.7K IR- Leishmania major strain LT252 1.7K
                     intergenic region"
     misc_feature    8296..9381
                     /note="3' SSU- segment containing 3' region of Leishmania
                     major strain Friedlin small subunit ribosomal RNA"
     promoter        complement(9398..9416)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9437..9453)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9461..9477
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9485..9515)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9530..9551)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9839..10427)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10601..11458)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(11459..11563)
                     /label=AmpR promoter
     rep_origin      11590..12045
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     12186..12202
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        12212..12230
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    12244..13156
                     /note="5' SSU- segment containing 5' region of Leishmania
                     major strain Friedlin small subunit ribosomal RNA"
     misc_feature    13185..13417
                     /note="DST IR- Leishmania major strain DST intergenic
                     region"
     misc_feature    13340..13341
                     /label=SA- spice acceptor AG dinucleotide
                     /note="SA- spice acceptor AG dinucleotide"
ORIGIN
        1 cctggtcgct agattaatta aatcaccact ttgtacaaga aagctgaacg agaaacgtaa
       61 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatgc
      121 tgtaaaacac aacatatcca gtcactatgg tcgacctgca gactggctgt gtataaggga
      181 gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg
      241 gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg ccatatcggt
      301 ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt cacgggagac
      361 tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc cgggcgtgtc
      421 aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt tataggtgta
      481 aaccttaaac tgcatttcac cagcccctgt tctcgtcagc aaaagagccg ttcatttcaa
      541 taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt tcggcacgca
      601 gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg acatcatata
      661 tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct gcttcatagc
      721 atacctcttt ttgacatact tcgggtatac atatcagtat atattcttat accgcaaaaa
      781 tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc acgcgtttac
      841 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
      901 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
      961 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
     1021 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
     1081 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     1141 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     1201 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     1261 tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     1321 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     1381 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     1441 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     1501 gcttccttag ctcctgaaaa tctcgacgga tcctaactca aaatccacac attatacgag
     1561 ccggaagcat aaagtgtaaa gcctggggtg cctaatgcgg ccgccaatat gactggatat
     1621 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
     1681 tatttatatc attttacgtt tctcgttcag cttttttgta caaacttgtg atagatcttc
     1741 tagctccccg agtggaccag gaaaagtggg cacagcgttc attcagatac tatctgtttt
     1801 ccctcattat gaatctgagc cgtgatgctt atgagattcg cctattgatg gaacaagaga
     1861 cttcagctta cagcaggcga atgaaggttt ctggagtagg agtctcagga ggagtagaaa
     1921 ctgtgggacc tggaggacca gggactccag gaggaagtct gccacaactg gctttgaagt
     1981 ttcggctccg aatcctgcta ctggctcgtg tccttcgagg tcatccccct ctcttgctgg
     2041 atgtgctcag aaatgcctgt gaccttttca tcccactgga caaactaggc ctctggcgtt
     2101 gtggccctgg gattgtgggg ctgtgtggcc tcatatcctc cattctgtct attctcaccc
     2161 taatctgtcc ctggctacga ctcaagcccg gatccgccgc tctgagcaaa agctcatttc
     2221 tgaagaggac ttgaatggag aacagaaatt gatcagtgag gaagacctca acggtgagca
     2281 gaagttaata tccgaggagg atcttcctag aagatctatc acaagtttgt acaaaaaagc
     2341 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     2401 acagactaca taatactgta aaacacaaca tatccagtca tattggcggc cgcattaggc
     2461 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
     2521 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     2581 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     2641 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     2701 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     2761 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     2821 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     2881 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     2941 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     3001 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     3061 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     3121 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     3181 cagtactgcg atgagtggca gggcggggcg taaacgcgtg gatccggctt actaaaagcc
     3241 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
     3301 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
     3361 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
     3421 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
     3481 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
     3541 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
     3601 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
     3661 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
     3721 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
     3781 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
     3841 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
     3901 catagtgact ggatatgttg tgttttacag cattatgtag tctgtttttt atgcaaaatc
     3961 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
     4021 gtggtgattt aattaatcta ggccaagctg atctatgagt cttgtgatgt actggctgat
     4081 ttctacgacc agttcgctga ccagttgcac gagtctcaat tggacaaaat gccagcactt
     4141 ccggctaaag gtaacttgaa cctccgtgac atcttagagt cggacttcgc gttcgcgtaa
     4201 cgccaaatca atacgacccg gatctccctt tagtgagggt taattagtcc tgcattaatg
     4261 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg ctgggcttaa
     4321 gggagcagac tcgtgcctcg cactgatgat gtagatgcac gcgtgcgtgt ggtgcgcggg
     4381 accgccgcca cggcgcttgt gtgccggtgt gtggcgaccg tctcggggaa ggaggaggag
     4441 cgcgcgtgcg tgtgctgcgc ctcctctttt ttctccctca ccgggttttt ttctgagggg
     4501 acgggggggg gaggaggagg cgcggatggc ggagcggcca cgctctgccc ctcgctgccc
     4561 tgtgcctttt ctcggccccc tccccgccct ctccttcctc gtgcacgtct gccgcgcatg
     4621 cacacgcgcg cgttgccgta gtgctgtacc ttcatggaaa aggagagcct cgcctctccc
     4681 tctctgtcgc tgatagacga atgagaaaag ggggagagca gtgcgcacgc gtgcaggcgg
     4741 attcgagcac gtgggcacgc tccgttggat gtgtcgtgcg gcttggggga atgtagcgac
     4801 gcgcttctcg cactgagaca ccaccactgt ctcccggtgt tgctgcactg ctgctgctgc
     4861 tgccacgtgg gcggacacct gccatgcgtc tcctcctctc accgtgccct ttttctcctg
     4921 ccctcttcgc gaaaacgaaa catgcgcaac gcacacacgc acccacaccc gcagtccttc
     4981 actctcgttc ttcgaacaaa caccctaaac cgccttctaa cctctctttc tttttctcca
     5041 accatcgata agatctccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     5101 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     5161 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     5221 ctgcccgtgc cctggcccac cctcgtgacc accttcacct acggcgtgca gtgcttcagc
     5281 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     5341 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     5401 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     5461 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     5521 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     5581 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     5641 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     5701 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     5761 atggacgagc tgtacaagta aagatcttat cgataagatc tatgagtctt gtgatgtact
     5821 ggctgatttc tacgaccagt tcgctgacca gttgcacgag tctcaattgg acaaaatgcc
     5881 agcacttccg gctaaaggta acttgaacct ccgtgacatc ttagagtcgg acttcgcgtt
     5941 cgcgtaacgc caaatcaata cgacccggat ctccctttag tgagggttaa ttagtcctgc
     6001 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgcta
     6061 ctcgggtgtc gcacacactg taaaacgccc ccgccggctc tgtcacgcaa gaaacgagag
     6121 caaaaagacc ggtagactat atcacgcaca atcaccgcgt gtgcgtctcc ctgggtgaag
     6181 acacccatcg cacccttcga cagccgccct tatgcctatt caccgtctgt agaacacaca
     6241 agaggaatag cccggtgccg cgtgcaagac tgcggcttct gcacgcacta tgctcgtttc
     6301 cgcctctctc tctttgtgcg cgtgtgtgtg tgtgtgtcgg agtggccctc ccgttacgtc
     6361 ttttgggggt gggtgatagc ggcagatgct gcttcgacct tgtgcgccgc accggtgccg
     6421 ttggctacac tgcggaaggc aacacagaac acaccctgtg ccatttcttc tttttttttt
     6481 gctttcaccc accttttccc cgtgcttccc catctttccc cctctttccc taacgtacat
     6541 tgcacctctc cttatcgtgc agtcacacgc taccactcaa cgctccctgc aacactggag
     6601 tgagtcgcta gaaataattt tgtttaactt taagaaggag atatacatag tgaccggatc
     6661 cccaccatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     6721 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     6781 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     6841 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     6901 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     6961 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     7021 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     7081 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     7141 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     7201 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     7261 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     7321 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     7381 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     7441 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     7501 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     7561 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     7621 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     7681 gcaaaggaat agactagttc tagttctagg gggcgcgaat tcagatcctc gtgtgagcgt
     7741 tcgcggaatc ggtcgctcgt gtttatgccc gtcttggtgt tgtgctcgca aggcggtgca
     7801 gcaggatacc gtcgccctcc tctctccttg cttctctgtt cttcaattcg cgatctcaca
     7861 gaggccggct gtgcacgccc ttcctcaccc tccttttccc acctctcggc caccggtcgg
     7921 ctccgttccg tctgccgtcg agaagggacg ggcatgtgca gctcctccct ttctctcgcg
     7981 cgcgcatctt ctcttgcttg tggcactcac gctcatgcgt caaggcggcc ccacgcgagc
     8041 ccctgcgctc ccttccctct tgcgcatccg tagccggact ggtcgatgcg caaggccggc
     8101 atgaaggagc gcgtgccctc aagagcggac tatcatgccc tacgtgggcc acgcagcgat
     8161 gaggccggct tcgcggagat gcgtcacgca cgtgccagat gatgccgtac gccttccttg
     8221 acttgcgccc ccctctcttc ctccgtctct cactctctct ctctcacaca cacacacaca
     8281 cacacacaca cacaaagctc cggttcgtcc ggccgtaacg ccttttcaac tcacggcctc
     8341 taggaatgaa ggagggtagt tcgggggaga acgtactggg gcgtcagagg tgaaattctt
     8401 agaccgcacc aagacgaact acagcgaagg cattcttcaa ggataccttc ctcaatcaag
     8461 aaccaaagtg tggagatcga agatgattag agaccattgt agtccacact gcaaacgatg
     8521 acacccatga attggggatc ttatgggccg gcctgcggca gggtttaccc tgtgtcagca
     8581 ccgcgcccgc ttttaccaac ttacgtatct tttctattcg gcctttaccg gccacccacg
     8641 ggaatatcct cagcacgttt tctgtttttt cacgcgaaag ctttgaggtt acagtctcag
     8701 gggggagtac gttcgcaaga gtgaaactta aagaaattga cggaatggca ccacaagacg
     8761 tggagcgtgc ggtttaattt gactcaacac ggggaacttt accagatccg gacaggatga
     8821 ggattgacag attgagtgtt ctttctcgat tccctgaatg gtggtgcatg gccgcttttg
     8881 gtcggtggag tgatttgttt ggttgattcc gtcaacggac gagatccaag ctgcccagta
     8941 gaattcagaa ttgcccatag aatagcaaac tcatcggcgg gttttaccca acggtgggcc
     9001 gcattcggtc gaattcttct ctgcgggatt cctttgtaat tgcacaaggt gaaattttgg
     9061 gcaacagcag gtctgtgatg ctcctcaatg ttctgggcga cacgcgcact acaatgtcag
     9121 tgagaacaag aaaaacgact tttgtcgaac ctacttgatc aaaagagtgg ggaaaccccg
     9181 gaatcacata gacccacttg ggaccgagga ttgcaattat tggtcgcgca acgaggaatg
     9241 tctcgtaggc gcagctcatc aaactgtgcc gattacgtcc ctgccatttg tacacaccgc
     9301 ccgtcgttgt ttccgatgat ggtgcaatac aggtgatcgg acaggcggtg ttttatccgc
     9361 ccgaaagttc accgatattt aaatccagct tttgttccct ttagtgaggg ttaattgcgc
     9421 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     9481 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     9541 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     9601 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     9661 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     9721 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     9781 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9841 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9901 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9961 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    10021 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    10081 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    10141 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    10201 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    10261 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10321 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    10381 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    10441 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10501 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10561 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10621 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10681 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10741 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10801 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10861 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10921 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10981 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    11041 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    11101 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    11161 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    11221 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    11281 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    11341 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    11401 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    11461 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11521 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11581 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
    11641 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
    11701 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
    11761 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
    11821 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
    11881 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
    11941 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
    12001 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttccatt cgccattcag
    12061 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    12121 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    12181 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattgga
    12241 tttaaattgg ataacttggc gaaacgccaa gctaatacat gaaccaaccg ggtgttctcc
    12301 actccagacg gtgggcaacc atcgtcgtga gacgcccagc gaatgaatga cagtaaaacc
    12361 aatgccttca ctggcagtaa cacccagcag tgttgactca attcattccg tgcgaaagcc
    12421 ggcttgttcc ggcgtctttt gacgaacaac tgccctatca gctggtgatg gccgtgtagt
    12481 ggactgccat ggcgttgacg ggagcggggg attagggttc gattccggag agggagcctg
    12541 agaaatagct accacttcta cggagggcag caggcgcgca aattgcccaa tgtcaaaaca
    12601 aaacgatgag gcagcgaaaa gaaatagagt tgtcagtcca tttggattgt catttcaatg
    12661 ggggatattt aaacccatcc aatatcgagt aacaattgga ggacaagtct ggtgccagca
    12721 cccgcggtaa ttccagctcc aaaagcgtat attaatgctg ttgctgttaa agggttcgta
    12781 gttgaactgt gggctgtgca ggtttgttcc tggtcgtccc gtccatgtcg gatttggtga
    12841 cccaggccct tgcagcccgt gaacattcaa agaaacaaga aacacgggag tggttccttt
    12901 cctgatttac gcatgtcatg catgccaggg ggcgtccgtg attttttact gtgactaaag
    12961 aagcgtgact aaagcagtca tttgacttga attagaaagc atgggataac aaaggagcag
    13021 cctctaggct accgtttcgg cttttgttgg ttttaaaggt ctattggaga ttatggagct
    13081 gtgcgacaag tgctttccca tcgcaacttc ggttcggtgt gtggcgcctt tgaggggttt
    13141 agtgcgtccg gtgcgatagg gagaccacaa cggtttccct ctagtgcgtg aagggttacc
    13201 gcaacgatgc gcaatggact cccccgcttt ccatttcgtc accttccgcc tctctctctc
    13261 tctctctcac catctacgcg tgcacatcat caactgtctc ttgtcggtgc tcaccaccct
    13321 caaccacccc tcactttcaa ggcttcccga acgcacacaa aaggcgtgaa aaccgctcgc
    13381 gtgtgttgag ccgtccaccg tagccctccc cctgtcc
//
